Luminol-amplified chemiluminescence by human polymorphonuclear leukocytes

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Intra- and extracellular events in luminol-dependent chemiluminescence of polymorphonuclear leukocytes.

When polymorphonuclear leukocytes (PMNL) and soluble or particulate matter interact, the cells produce chemiluminescence. Luminol-dependent light emission from PMNL is linked to the myeloperoxidase (MPO)-H2O2 system. Light emission from a cell-free MPO-H2O2 system was found to be totally inhibited by human serum albumin (HSA), and since HSA is a large molecular protein that does not readily gai...

متن کامل

Human Polymorphonuclear Leukocytes by Retinoic Acid and Inhibition of Phorbol Ester-stimulated Chemiluminescence in

Chemiluminescence (CL) is an index of both the generation of and reactions mediated by Cv and 'O2. The tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA), is a potent stim ulator of CL by human polymorphonuclear leukocytes; treat ment with TPA (100 ng/ml) provokes a CL response that peaks within five min and persists for over 30 min. The response is proportional to concentration over th...

متن کامل

Human Polymorphonuclear Leukocytes

A B S T R A C T Acetyl glyceryl ether phosphorylcholine (AGEPC) and leukotriene B4 (LTB4) induce concentration-dependent neutrophil aggregation. On a molar basis, LTB4 is 10 to 100 times more potent than AGEPC. AGEPC-induced aggregation is attenuated by two inhibitors of arachidonate lipoxygenation, eicosatetraynoic acid and nordihydroguaiaretic acid, and to a lesser extent by the cyclooxygenas...

متن کامل

Arachidonate metabolism by human polymorphonuclear leukocytes

Release of arachidonic acid by the membrane phospholipase and metabolism by the 5-lipoxygenase pathway was examined in human polymorphonuclear leukocytes (PMNs). The 5-lipoxygenase pathway is activated when PMNs are given arachidonic acid in ethanol and there is extensive metabolism to 5-hydroxyicosatetraenoic acid (5-HETE) and leukotriene B4 (LTB4). This activation event was shown to be altere...

متن کامل

Chemotaxis by polymorphonuclear leukocytes

The ability of a cell or organism to direct its movement along a chemical gradient has fascinated biologists for over 100 yr. This process of chemotaxis requires transformation of directional information from the environment into a series of cellular responses resulting in directional movement. Many lower organisms including bacteria, protozoa, and slime molds exhibit chemotaxis. This ability h...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Ensho

سال: 1983

ISSN: 0389-4290,1884-4006

DOI: 10.2492/jsir1981.3.supplement_82